Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: DC-SIGN is a receptor for human herpesvirus 8 on dendritic cells and macrophages.

doi: 10.4049/jimmunol.176.3.1741

Figure Lengend Snippet: FIGURE 3. Infection of DCs with HHV-8 is blocked by anti DC-SIGN mAb. A, Immunofluorescence results on DCs that were left untreated or treated with either anti-DC-SIGN mAb (clone 120507) or anti-CD11a mAb, infected with HHV-8, incubated, and stained with anti-K8.1A/B mAb (red) at 24 h. Uninfected DCs were used as controls. B, Immunoflu- orescence results on DCs that were either treated with anti-DC-SIGN mAb (clone 120507) or left untreated, infected, and stained at 24 h with anti- DC-SIGN mAb (green) and anti-ORF 59 mAb (red). The overlay of com- bined colors for anti-DC-SIGN and ORF 59 is shown. Cells were coun- terstained with DAPI (blue) (600). Data are from one experiment representative of eight independent experiments.

Article Snippet: For blocking studies, cells were pretreated with 20 g/ml anti-human-DC-SIGN mAb (clone 120507; R&D Systems, or clone DCN46; BD Biosciences), anti-CD11a mAb (BD Biosciences), mouse IgG (Sigma-Aldrich), or 100 g/ml mannan (Sigma-Aldrich), for 1 h at 4°C before exposure to HHV-8.

Techniques: Infection, Incubation, Staining

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: DC-SIGN is a receptor for human herpesvirus 8 on dendritic cells and macrophages.

doi: 10.4049/jimmunol.176.3.1741

Figure Lengend Snippet: FIGURE 4. DC-SIGN expression renders resistant cells susceptible to HHV-8 infection. A, Immunofluorescence results on K562 and K562-DC- SIGN cells that were infected with HHV-8 and stained with anti-K8.1A/B mAb at 24 h (red). B, Immunofluorescence results on B-LCL and B-LCL DC-SIGN that were infected with HHV-8 and stained after 24 h with anti- K8.1A/B mAb (red). Cells were counterstained with DAPI (600). Data are from one experiment representative of five independent experiments.

Article Snippet: For blocking studies, cells were pretreated with 20 g/ml anti-human-DC-SIGN mAb (clone 120507; R&D Systems, or clone DCN46; BD Biosciences), anti-CD11a mAb (BD Biosciences), mouse IgG (Sigma-Aldrich), or 100 g/ml mannan (Sigma-Aldrich), for 1 h at 4°C before exposure to HHV-8.

Techniques: Expressing, Infection, Staining

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: DC-SIGN is a receptor for human herpesvirus 8 on dendritic cells and macrophages.

doi: 10.4049/jimmunol.176.3.1741

Figure Lengend Snippet: FIGURE 5. HHV-8 binds to DC-SIGN. A, Inhibition of binding of radio- actively labeled HHV-8 by treatment of target cells with anti-DC-SIGN mAb. DCs or B-LCL-DC-SIGN were pretreated with anti-DC-SIGN mAb (clone 120507) or mannan, or left untreated. Each bar represents the mean percent of binding inhibition ( SE) (compared with untreated cells) from two duplicate determinations. B, Inhibition of binding of radioactively labeled HHV-8 by treatment of virus with soluble DC-SIGN. Results are the mean ( SE) per- centage of inhibition of binding of soluble DC-SIGN-treated HHV-8 com- pared with binding of radiolabeled untreated virus to each cell type from two determinations. C, Dose response of inhibition of virus binding to DCs by treatment with anti-DC-SIGN mAb. Each bar represents the mean of duplicate reactions ( SE) from duplicate determinations. Data are from one experiment representative of three independent experiments.

Article Snippet: For blocking studies, cells were pretreated with 20 g/ml anti-human-DC-SIGN mAb (clone 120507; R&D Systems, or clone DCN46; BD Biosciences), anti-CD11a mAb (BD Biosciences), mouse IgG (Sigma-Aldrich), or 100 g/ml mannan (Sigma-Aldrich), for 1 h at 4°C before exposure to HHV-8.

Techniques: Inhibition, Binding Assay, Labeling, Virus

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: DC-SIGN is a receptor for human herpesvirus 8 on dendritic cells and macrophages.

doi: 10.4049/jimmunol.176.3.1741

Figure Lengend Snippet: FIGURE 6. HHV-8 infection of IL-13-activated macrophages is related to DC-SIGN expression. A, Flow cytometric analysis showing expression of DC-SIGN on HHV-8-infected (empty histogram, broken line) or un- infected (empty histogram, solid line) IL-13-activated macrophages. Full histogram, isotype controls. B, Im- munofluorescence results on IL-13-activated macro- phages that were infected with HHV-8 for 24 h and stained for ORF 59 (red) and DC-SIGN (green). The overlay of combined colors for anti-DC-SIGN and ORF59 is shown. C, Immunofluorescence results on IL- 13-activated macrophages that were pretreated with anti- DC-SIGN mAb (clone 120507) or mouse IgG, infected with HHV-8 for 24 h and stained for anti-K8.1 mAb (red). Cells were counterstained with DAPI (600). Data are from one experiment representative of four in- dependent experiments.

Article Snippet: For blocking studies, cells were pretreated with 20 g/ml anti-human-DC-SIGN mAb (clone 120507; R&D Systems, or clone DCN46; BD Biosciences), anti-CD11a mAb (BD Biosciences), mouse IgG (Sigma-Aldrich), or 100 g/ml mannan (Sigma-Aldrich), for 1 h at 4°C before exposure to HHV-8.

Techniques: Infection, Expressing, Staining

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: DC-SIGN is a receptor for human herpesvirus 8 on dendritic cells and macrophages.

doi: 10.4049/jimmunol.176.3.1741

Figure Lengend Snippet: FIGURE 7. Effect of HHV-8 in- fection of DCs on expression of DC- SIGN and costimulatory molecules. A, DC-SIGN expression on unin- fected or HHV-8 infected DCs. Data are mean MFI (SE) from seven in- dependent experiments. B, Expres- sion of HLA-ABC, HLA-DR, CD83, and DC-SIGN on infected DCs. Blue histogram, HHV-8-infected DCs; yellow histogram, uninfected DCs; empty histogram, fine line, and bro- ken line isotype controls for the infected and uninfected DCs, respec- tively. Data are from one experiment representative of 10 independent ex- periments. C, Confocal microscopy of HHV-8-infected DCs stained with anti-DC-SIGN (green) and anti-ORF 59 (red) mAbs at 24 h (left panel) and 48 h (center panel) after infection. Uninfected DCs served as controls (right panel). Data are from one ex- periment representative of two inde- pendent experiments.

Article Snippet: For blocking studies, cells were pretreated with 20 g/ml anti-human-DC-SIGN mAb (clone 120507; R&D Systems, or clone DCN46; BD Biosciences), anti-CD11a mAb (BD Biosciences), mouse IgG (Sigma-Aldrich), or 100 g/ml mannan (Sigma-Aldrich), for 1 h at 4°C before exposure to HHV-8.

Techniques: Expressing, Infection, Confocal Microscopy, Staining

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Internalizing antibodies to the C-type lectins, L-SIGN and DC-SIGN, inhibit viral glycoprotein binding and deliver antigen to human dendritic cells for the induction of T cell responses.

doi: 10.4049/jimmunol.176.1.426

Figure Lengend Snippet: FIGURE 1. Screening of phage-displayed Fabs for L-SIGN reactivity. A, Ninety-six Fab phage clones selected from three rounds of pan- ning on recombinant DC-SIGN (negative selec- tion) and L-SIGN (positive selection) proteins were screened for binding to K562 (negative control) and K562/L-SIGN-transfected cells by flow cytometry using goat anti-mouse IgG PE conjugate for detection. Only clones showing at least a 5-fold higher binding to K562/L-SIGN cells are shown. B, To select Fab phage clones uniquely reactive with L-SIGN, but not DC- SIGN, K562/L-SIGN cell-reactive clones were screened for reactivity with DC-SIGN-Fc and L- SIGN-Fc fusion proteins in a capture ELISA. mAb162 (L-SIGN specific), mAb1621, and mAb16211 (DC-SIGN/L-SIGN cross-reactive) were used as positive controls. Data are repre- sentative of two independent experiments.

Article Snippet: Before their use in assays, RBC were lysed and any remaining dead cells were further depleted using a dead cell removal kit (Miltenyi Biotec; catalog 130-090-101), per the manufacturer’s instructions. mAbs mAb162 (reactive only with L-SIGN), mAb1621, and mAb16211 (cross-reactive with DC-SIGN and L-SIGN) were purchased from R&D Systems.

Techniques: Clone Assay, Recombinant, Selection, Binding Assay, Negative Control, Transfection, Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Molecular and Cellular Biochemistry

Article Title: Low expression of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein in lung cancer and significant correlations with brain metastasis and natural killer cells

doi: 10.1007/s11010-015-2465-4

Figure Lengend Snippet: Clinical data of the lung cancer patients in ELISA study

Article Snippet: Each plate was incubated at 37 °C for 2 h. Subsequently, PBST was used to wash the plates 3 times, and 100 μl anti-DC-SIGNR mouse monoclonal antibody (mAb, R&D Systems, Inc., USA, Catalog Number: MAB16211) diluted to a concentration of 0.5 μg/ml was added to each wells.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Molecular and Cellular Biochemistry

Article Title: Low expression of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein in lung cancer and significant correlations with brain metastasis and natural killer cells

doi: 10.1007/s11010-015-2465-4

Figure Lengend Snippet: Low serum levels of DC-SIGNR in lung cancer patients. The scatter plot displays DC-SIGNR levels in serum samples from 173 lung cancer patients and 134 healthy controls were tested by enzyme-linked immunosorbent assay (ELISA). Each point represents the serum DC-SIGNR level of one sample. The horizontal lines indicate the median levels. Serum DC-SIGNR was significantly lower in lung cancer patients compared with healthy controls ( P = 0.0003) ( a ). Receiver operating characteristic (ROC) curve was used for serum DC-SIGNR in lung cancer prediction. A cut-off value of 3.8998 ng/L for DC-SIGNR to predict the presence of lung cancer with 78.03 % sensitivity and 49.25 % specificity (Area under curve (AUC) = 0.6212, P = 0.0003) ( b )

Article Snippet: Each plate was incubated at 37 °C for 2 h. Subsequently, PBST was used to wash the plates 3 times, and 100 μl anti-DC-SIGNR mouse monoclonal antibody (mAb, R&D Systems, Inc., USA, Catalog Number: MAB16211) diluted to a concentration of 0.5 μg/ml was added to each wells.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Molecular and Cellular Biochemistry

Article Title: Low expression of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein in lung cancer and significant correlations with brain metastasis and natural killer cells

doi: 10.1007/s11010-015-2465-4

Figure Lengend Snippet: Serum DC-SIGNR levels were significantly correlated with brain metastasis. The serum levels of DC-SIGNR were not significantly different between metastasis and non-metastasis ( P = 0.3887) ( a ). Serum concentrations of DC-SIGNR were higher in brain metastasis than in non-metastasis ( P = 0.0283) ( b ). However, the serum DC-SIGNR levels were not significantly different between bone metastasis and non-metastasis ( P = 0.3841), lung metastasis and non-metastasis ( P = 0.6744), lymph node metastasis and non-metastasis ( P = 0.9880) ( b )

Article Snippet: Each plate was incubated at 37 °C for 2 h. Subsequently, PBST was used to wash the plates 3 times, and 100 μl anti-DC-SIGNR mouse monoclonal antibody (mAb, R&D Systems, Inc., USA, Catalog Number: MAB16211) diluted to a concentration of 0.5 μg/ml was added to each wells.

Techniques:

Journal: Molecular and Cellular Biochemistry

Article Title: Low expression of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein in lung cancer and significant correlations with brain metastasis and natural killer cells

doi: 10.1007/s11010-015-2465-4

Figure Lengend Snippet: Correlations of serum levels of DC-SIGNR with NK, CD3, CD4, and CD8 levels. Serum DC-SIGNR levels correlated significantly with percent of NK cells in lung cancer patients ( P = 0.0017) ( a ). Serum levels of DC-SIGNR showed no significant correlation with CD3 ( b ), CD4 ( c ), and CD8 ( d )

Article Snippet: Each plate was incubated at 37 °C for 2 h. Subsequently, PBST was used to wash the plates 3 times, and 100 μl anti-DC-SIGNR mouse monoclonal antibody (mAb, R&D Systems, Inc., USA, Catalog Number: MAB16211) diluted to a concentration of 0.5 μg/ml was added to each wells.

Techniques:

Journal: Molecular and Cellular Biochemistry

Article Title: Low expression of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein in lung cancer and significant correlations with brain metastasis and natural killer cells

doi: 10.1007/s11010-015-2465-4

Figure Lengend Snippet: Correlations of serum levels of DC-SIGNR with clinical data. Serum levels of DC-SIGNR showed no significant correlation with gender ( a ), age ( b , c ), cancer stage ( d ), and histologic subtype ( e , f )

Article Snippet: Each plate was incubated at 37 °C for 2 h. Subsequently, PBST was used to wash the plates 3 times, and 100 μl anti-DC-SIGNR mouse monoclonal antibody (mAb, R&D Systems, Inc., USA, Catalog Number: MAB16211) diluted to a concentration of 0.5 μg/ml was added to each wells.

Techniques:

Journal: Molecular and Cellular Biochemistry

Article Title: Low expression of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein in lung cancer and significant correlations with brain metastasis and natural killer cells

doi: 10.1007/s11010-015-2465-4

Figure Lengend Snippet: Correlations of serum levels of DC-SIGNR with tumor markers. Serum levels of DC-SIGNR displayed no significant correlation with S100β ( a ) CEA ( b ), NSE ( c ), and CYFRA21-1 ( d )

Article Snippet: Each plate was incubated at 37 °C for 2 h. Subsequently, PBST was used to wash the plates 3 times, and 100 μl anti-DC-SIGNR mouse monoclonal antibody (mAb, R&D Systems, Inc., USA, Catalog Number: MAB16211) diluted to a concentration of 0.5 μg/ml was added to each wells.

Techniques:

Journal: Molecular and Cellular Biochemistry

Article Title: Low expression of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein in lung cancer and significant correlations with brain metastasis and natural killer cells

doi: 10.1007/s11010-015-2465-4

Figure Lengend Snippet: IHC for DC-SIGNR expression in tissues of lung cancer tissues, tuberculosis patients, and normal tissues. IHC analysis was used to determine DC-SIGNR expression and arrows indicate positive staining. Areas in the black boxes of A, C, E, G, and I were enlarged below. DC-SIGNR expression was detected in lung tissues from tuberculosis patients ( a – b ). DC-SIGNR expression was detected in the lung tissues from lung cancer patients ( c – d ). DC-SIGNR expression in alveolar epithelia cells of normal lung tissues ( e – f ). DC-SIGNR expression in lymphatic endothelial cells exhibited strong positive ( g – h ). Normal lymph node tissues were used as a negative control ( i–j )

Article Snippet: Each plate was incubated at 37 °C for 2 h. Subsequently, PBST was used to wash the plates 3 times, and 100 μl anti-DC-SIGNR mouse monoclonal antibody (mAb, R&D Systems, Inc., USA, Catalog Number: MAB16211) diluted to a concentration of 0.5 μg/ml was added to each wells.

Techniques: Expressing, Staining, Negative Control

Journal: Molecular and Cellular Biochemistry

Article Title: Low expression of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein in lung cancer and significant correlations with brain metastasis and natural killer cells

doi: 10.1007/s11010-015-2465-4

Figure Lengend Snippet: Semi-quantitative image analysis of DC-SIGNR expression in tissues. The graph displays a scatter plot of the levels of DC-SIGNR expression in lung tissues from lung cancer patients, tuberculosis patients, and normal controls. There was a statistical significance in IHC for DC-SIGNR expression between lung cancer tissues and normal lung tissues ( P = 0.0418), tuberculosis tissues and normal controls ( P = 0.0289). There was no significantly difference between lung cancer patients and tuberculosis patients ( P = 0.2696). DC-SIGNR expression in lung cancer patients was lower than in normal lung tissues

Article Snippet: Each plate was incubated at 37 °C for 2 h. Subsequently, PBST was used to wash the plates 3 times, and 100 μl anti-DC-SIGNR mouse monoclonal antibody (mAb, R&D Systems, Inc., USA, Catalog Number: MAB16211) diluted to a concentration of 0.5 μg/ml was added to each wells.

Techniques: Expressing

Journal: Glycovirology Protocols

Article Title: The C Type Lectins DC-SIGN and L-SIGN

doi: 10.1007/978-1-59745-393-6_4

Figure Lengend Snippet: Structure of DC-SIGN and L-SIGN proteins. The C-type lectins DC-SIGN and L-SIGN are type II transmembrane proteins. Their cytoplasmic tails contain internalization signals (di-leucine, tyrosine, and tri-acidic) which are involved in internalization of the lectin. The extracellular domain is composed of a carbohydrate recognition domain (CRD) and a neck domain (conserved in the case of DC-SIGN, variable for L-SIGN) implicated in the oligomerization of these lectins. The oligomerization is probably important for the orientation and subsequently for the function of the CRDs.

Article Snippet: Phycoerythrin (PE)-conjugated mouse monoclonal antibodies (mAbs) directed against DC-SIGN (FAb161P), L-SIGN (FAb162P), or both lectins (FAb1621P) were purchased from R&D Systems.

Techniques: